Plastocyanin Silencing will Decrease PVXCP Accumulation in Chloroplasts of Tobacco Plant

Plastocyanin is an important component in photosynthesis and biogenesis of chloroplast. It is a nucleus-encoded protein and transports into chloroplast with the help of transit peptide. PVXCP interacts with plastocyanin transit peptide in the transportation of PVXCP into chloroplast. A research was conducted to investigate the interaction of plastocyanin and PVXCP. Virus induce gene silencing approach had been used to silent plastocyanin in tobacco plant from the beginning. Plastocyanin silencing mRNA was inserted on TRV2 plasmid of Tobacco mosaic virus. TRV1 and TRV2 were transformed into Agarobacteria separately and the clones will be agroinfiltrated into the young leaves. The efficiency of PVXCP transporting into chloroplast and amount of PVXCP accumulated in various parts of chloroplast in plastocynin-silenced plant was examined and confirmed by using western plot. In second part of this project, pBI121-PVXCP was cloned by using cloning method. The pBI121-PVXCP will express PVXCP without the presence of PVX in tobacco plant and level of PVXCP accumulated in chloroplast can be determined in future work.